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Scintillation Vial, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scintillation vials  (Revvity)
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Scintillation Vials, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scintillation vials - by Bioz Stars, 2026-04
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liquid scintillation vials  (Thermo Fisher)
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Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid <t>scintillation</t> counter.
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scintillation vials micronic vials  (Micronic Inc)
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Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid <t>scintillation</t> counter.
Scintillation Vials Micronic Vials, supplied by Micronic Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scintillation vials monovette edta  (Sarstedt)
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Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid <t>scintillation</t> counter.
Scintillation Vials Monovette Edta, supplied by Sarstedt, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plastic snaptwist scintillation vial s207  (Simport Scientific)
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Simport Scientific plastic snaptwist scintillation vial s207
Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid <t>scintillation</t> counter.
Plastic Snaptwist Scintillation Vial S207, supplied by Simport Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid scintillation counter.

Journal: STAR Protocols

Article Title: Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells

doi: 10.1016/j.xpro.2025.103935

Figure Lengend Snippet: Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid scintillation counter.

Article Snippet: Liquid scintillation vials , Thermo Fisher Scientific , Cat#S31.

Techniques: In Vitro, Binding Assay